In Silico Analysis and Tissue-Specific Transcription of Platyfish (Xiphophorus maculatus) Catalase Gene

The present study focused on conducting in silico analysis and investigating the tissue-specific distribution expression of catalase gene platyfish (Xiphophorus maculatus), which can be used as a model organism for studying stress responses fish. Assay steady-state levels cat transcripts by real time PCR revealed. level transcript was abundant liver (2162.21) compared with intestine (1270.94), heart (1241.25), muscle (419.157), brain (46.205), eye (47.57), swimming bladder (28.99), gills (81.18), spleen (95.45), kidney (20.25) ovary (91.16) testis (113.22). results suggest that is major site platyfish, significantly higher to other tissues. In addition, research involved using bioinformatics tools analyze genetic sequence predict its structure function. indicated Platyfish shares high identity similarity orthologs teleost species, including medaka, fugu, zebrafish. This observation suggests conserved among these fish gene’s function regulatory mechanisms are likely similar. conservation species highlights importance this antioxidant defense system potential role responding environmental stressors. exhibits structure, evidenced synteny orthologous cat/CAT genes humans. Overall, provides evidence highly contributes functional stability maintenance critical response mechanisms.